Anti-SOD2/MnSOD抗体
参阅全部 SOD2/MnSOD 一抗
兔多克隆抗体to SOD2/MnSOD
Rabbit
适用于: ICC/IF, IHC-P, IPmore details
与反应: Mouse, Rat, Human
Recombinant fragment corresponding to Rat SOD2/MnSOD.
Database link: P07895
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
This antibody was immunoaffinity purified.
多克隆
IgG
Abpromise™承诺保证使用ab13534于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use at an assay dependent concentration. |
| IHC-P | (2) | 1/100. |
| IP | Use a concentration of 10 µg/ml. |
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Unigene: 290876 Mouse
Unigene: 10488 Rat
Entrez Gene: 6648 Human
Entrez Gene: 20656 Mouse
Omim: 147460 Human
SwissProt: P04179 Human
SwissProt: P09671 Mouse
Unigene: 487046 Human
Manganese containing superoxide dismutase antibody
Manganese SOD antibody
Manganese superoxide dismutase antibody
Mangano superoxide dismutase antibody
Mn SOD antibody
Mn superoxide dismutase antibody
MNSOD antibody
MVCD6 antibody
SOD 2 antibody
SOD2 antibody
SODM_HUMAN antibody
Superoxide dismutase [Mn] mitochondrial antibody
Superoxide dismutase [Mn], mitochondrial antibody
Superoxide dismutase 2 mitochondrial antibody
Indophenoloxidase B antibody
IPO B antibody
IPOB antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13534)
ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13534)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification: 100x.
Left: DAPI (blue) nuclear stain. Middle: SOD2/MnSOD. Right: Merge.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (ab13534)This image is courtesy of an anonymous Abreview
ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.
Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (ab13534)This image is courtesy of an Abreview submitted by Dr Anne Chiaramello
ab13534 staining differentiating (rat) PC12 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 2 µg/ml. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary. Cells were counterstained with the nuclear TOPRO-3 stain.
Immunoprecipitation - Anti-SOD2/MnSOD antibody (ab13534)
Lane 1: ab13534 + Mouse brain lysate (250µg)
Lane 2: ab13534 + Mouse brain lysate (250µg, ½ dilution)
Lane 3: Mouse brain lysate (15µg)
Lane 4: Rat brain lysate (15µg)
Ab13534 at 1:1000 immunoprecipitating SOD2 in mouse brain and rat brain lysate. For western blotting, donkey anti- rabbit IgG (HRP) was used as the secondary antibody.
