Anti-RBM14抗体
参阅全部 RBM14 一抗
兔多克隆抗体to RBM14
Rabbit
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human RBM14 aa 150-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96PK6
Nuclear extract from HeLa cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab12325于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use. |
Entrez Gene: 100526737 Human
Entrez Gene: 10432 Human
Omim: 612409 Human
SwissProt: Q96PK6 Human
Unigene: 523822 Human
Unigene: 533712 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM14 antibody (ab12325)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-RBM14 antibody (ab12325)
Detection of Human CoAA by Western Blot.
Samples: Nuclear extract (NE) from HeLa cells.
Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human CoAA by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
