Anti-Casein Kinase 2 beta (phospho S209)抗体
参阅全部 Casein Kinase 2 beta 一抗
兔多克隆抗体to Casein Kinase 2 beta (phospho S209)
Rabbit
Does not cross react with Casein Kinase 2 alpha [pS360] (mouse), [pS362], [pS370], or Casein Kinase 2 beta pS2].
适用于: WBmore details
与反应: Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
HeLa cell lysates or K562 cell lysates.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
浓度
50 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Casein Kinase 2 beta. The final product is generated by affinity chromatography using a Casein Kinase 2 beta derived peptide that is phosphorylated at serine 209.
多克隆
IgG
Abpromise™承诺保证使用ab12861于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
应用说明
WB: 1/1000 in 3% milk in 0.1% Tween20 (TBST). Incubate overnight. Detects a band of approximately 25 kDa. Block 1hr with 3% milk in 0.1% Tween20 (TBST).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 1460 Human
Omim: 115441 Human
SwissProt: P67870 Human
Unigene: 73527 Human
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Western blot - Anti-Casein Kinase 2 beta (phospho S209) antibody (ab12861)
Peptide Competition and Phosphatase Treatment
Lysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with ab12861 antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method.
The data show that only the peptide corresponding to ab12861 blocks the signal, verifying the specificity of the antibody.
