Anti-KMT1A / SUV39H1 antibody [44.1]

• 产品名称

  Anti-KMT1A / SUV39H1抗体[44.1]
  参阅全部 KMT1A / SUV39H1 一抗

• 描述

  小鼠单克隆抗体[44.1] to KMT1A / SUV39H1

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 表位

  ab12405 recognizes an epitope in the N-terminal (195 amino acids) of human KMT1A / SUV39H1.

• 阳性对照

• HCT116, U87-MG and TE671 cell lysates.

• 常规说明

  Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  44.1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab12405于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 9244 Mouse

• Entrez Gene: 6839 Human

• Entrez Gene: 20937 Mouse

• Entrez Gene: 302553 Rat

• Omim: 300254 Human

• SwissProt: O43463 Human

• SwissProt: O54864 Mouse

• Unigene: 522639 Human

• Unigene: 479743 Mouse

• 别名

• Histone H3-K9 methyltransferase1 antibody

• Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody

• Histone-lysine N-methyltransferase SUV39H1 antibody

• KMT1 A antibody

• KMT1A antibody

• Lysine N methyltransferase 1A antibody

• Lysine N-methyltransferase 1A antibody

• MG44 antibody

• mIS6 antibody

• Position-effect variegation 3-9 homolog antibody

• Su(var)3 9 homolog 1 antibody

• Su(var)3-9 homolog 1 antibody

• Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody

• Suppressor of variegation 3-9 homolog 1 antibody

• SUV39 H1 antibody

• SUV39H antibody

• SUV39H1 antibody

• SUV91_HUMAN antibody

• H3 K9 HMTase1 antibody

• H3-K9-HMTase 1 antibody

• Histone H3-K9 methyltransferase 1 antibody

• 

  Western blot - Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405)

  All lanes : Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405) at 5 µg
  
  Lane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
  Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
  Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 48 kDa
  Observed band size: 60 kDawhy is the actual band size different from the predicted?
  Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 4 minutes
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab12405 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406