Anti-JAB1抗体
参阅全部 JAB1 一抗
兔多克隆抗体to JAB1
Rabbit
适用于: IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
Immunogen was a synthetic peptide, which represented a c-terminal domain of human JAB1 (LocusLink ID 10987).
Centrifuge tube to remove product from lid.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab12323于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/250 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | (2) | 1/5000 - 1/25000. Detects a band of approximately 33, 36 kDa (predicted molecular weight: 40 kDa). |
Entrez Gene: 10987 Human
Entrez Gene: 26754 Mouse
Entrez Gene: 444720 Xenopus laevis
Entrez Gene: 393698 Zebrafish
Omim: 604850 Human
SwissProt: Q92905 Human
SwissProt: O35864 Mouse
SwissProt: Q6GLM9 Xenopus laevis
SwissProt: Q6PC30 Zebrafish
Unigene: 491912 Human
Unigene: 402384 Mouse
Unigene: 3139 Xenopus laevis
Unigene: 81074 Zebrafish
38 kDa Mov34 homolog antibody
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COP9 constitutive photomorphogenic homolog subunit 5 antibody
COP9 signalosome complex subunit 5 antibody
COP9 signalosome subunit 5 antibody
Cop9 subunit 5 antibody
COPS 5 antibody
cops5 antibody
CSN 5 antibody
CSN5 antibody
CSN5_HUMAN antibody
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Jun activation domain binding protein 1 antibody
Jun activation domain binding protein antibody
Jun activation domain-binding protein 1 antibody
MGC3149 antibody
MOV 34 antibody
MOV34 antibody
MOV34 family, 38-KD member antibody
SGN 5 antibody
SGN5 antibody
Signalosome subunit 5 antibody
Western blot - Anti-JAB1 antibody (ab12323)This image is courtesy of an anonymous Abreview
All lanes : Anti-JAB1 antibody (ab12323) at 1/2500 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lane 3 : U2OS whole cell lysate
Lane 4 : Mouse embryonic fibroblast whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 35 kDawhy is the actual band size different from the predicted?
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (ab12323)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling JAB1 with ab12323 at 1/500 (2µg/ml). Detection: DAB.
Western blot - Anti-JAB1 antibody (ab12323)
Detection of Human CSN5 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure. Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
