Anti-IRF4抗体[MUM1p]
参阅全部 IRF4 一抗
小鼠单克隆抗体[MUM1p] to IRF4
Mouse
适用于: IHC-Pmore details
与反应: Human
Recombinant full length protein corresponding to Human IRF4.
Database link: Q15306
Tonsil.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA
Tissue culture supernatant
单克隆
MUM1p
IgG1
Abpromise™承诺保证使用ab12501于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
应用说明
IHC-P: 1/10 - 1/25. Perform heat mediated antigen retrieval with 1 mM EDTA buffer, pH 9.0 before commencing with IHC staining protocol. We suggest an incubation period of 30-60 minutes at room temperature.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 3662 Human
SwissProt: Q15306 Human
Unigene: 401013 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF4 antibody [MUM1p] (ab12501)
IHC image of ab12501 staining in human Hodgkin's lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12501, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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