Anti-Heme Oxygenase 1抗体
参阅全部 Heme Oxygenase 1 一抗
兔多克隆抗体to Heme Oxygenase 1
Rabbit
适用于: WB, ICC/IF, Sandwich ELISA, IHC-Pmore details
与反应: Mouse, Rat, Human
Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
200 µg 浓度为 1 mg/ml
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab13243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa). |
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) as Capture. Read More | |
| IHC-P | (3) | Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 3162 Human
Entrez Gene: 15368 Mouse
Omim: 141250 Human
SwissProt: P09601 Human
SwissProt: P14901 Mouse
Unigene: 517581 Human
Unigene: 276389 Mouse
Unigene: 3160 Rat
32 kD antibody
bK286B10 antibody
D8Wsu38e antibody
heat shock protein 32 kD antibody
heat shock protein 32kD antibody
Heat shock protein antibody
Heme oxygenase (decycling) 1 antibody
Heme oxygenase 1 antibody
Hemox antibody
HMOX 1 antibody
Hmox antibody
Hmox1 antibody
HMOX1_HUMAN antibody
HO 1 antibody
HO antibody
HO-1 antibody
HO1 antibody
Hsp32 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
Western blot - Anti-Heme Oxygenase 1 antibody (ab13243)
All lanes : Anti-Heme Oxygenase 1 antibody (ab13243) at 1/1000 dilution
Lane 1 : Hek293
Lane 2 : HL60
Lane 3 : HeLa
Lane 4 : A549
Lane 5 : Hu spleen
Lane 6 : Ms spleen
Lane 7 : Rt spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti Rabbit
Predicted band size: 34.6 kDa
Observed band size: 32 kDawhy is the actual band size different from the predicted?
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
IHC image of Heme Oxygenase 1 staining in formalin fixed, paraffin embedded rat spleen normal tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13243, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Heme Oxygenase 1 antibody (ab13243)
Anti-Heme Oxygenase 1 antibody (ab13243) (Rat microsomes)
Predicted band size: 34.6 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
Alzheimer diseased section stained with ab13243.
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody (ab13243)
ICC/IF image of ab13243 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13243, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Sandwich ELISA - Anti-Heme Oxygenase 1 antibody (ab13243)
Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.
