Anti-Myelin Basic Protein antibody [MBP2]

• 产品名称

  Anti-Myelin Basic蛋白抗体[MBP2]
  参阅全部 Myelin Basic Protein 一抗

• 描述

  小鼠单克隆抗体[MBP2] to Myelin Basic蛋白

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: Human brain tissue lysate. IHC-Fr: Mouse cerebrum tissue. IHC-P: Human, mouse and rat cerebrum tissue.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)

• 浓度

• 10 µg 浓度为 1.176 mg/ml

• 100 µg 浓度为 1.176 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  MBP2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11159于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 4155 Human

• Entrez Gene: 17196 Mouse

• Entrez Gene: 24547 Rat

• Omim: 159430 Human

• SwissProt: P02686 Human

• SwissProt: P04370 Mouse

• SwissProt: P02688 Rat

• Unigene: 551713 Human

• Unigene: 252063 Mouse

• Unigene: 392350 Mouse

• Unigene: 454459 Mouse

别名

• GDB antibody

• Golli MBP antibody

• Golli MBP; myelin basic protein antibody

• Hemopoietic MBP antibody

• HMBPR antibody

• HUGO antibody

• MBP antibody

• MBP_CAVPO antibody

• MBP_HUMAN antibody

• MGC99675 antibody

• MLD antibody

• Myelin A1 protein antibody

• Myelin A1 Protein, basic antibody

• Myelin basic protein antibody

• Myelin Deficient antibody

• Myelin membrane encephalitogenic protein antibody

• OTTHUMP00000163776 antibody

• OTTHUMP00000174387 antibody

• OTTHUMP00000174388 antibody

• SHI antibody

• Shiverer antibody

• SP antibody

• 

  Western blot - Anti-Myelin Basic Protein antibody [MBP2] (ab11159)

  Anti-Myelin Basic Protein antibody [MBP2] (ab11159) at 1/1000 dilution + human brain tissue lysate at 20 µg
  
  Secondary
  Anti-mouse IgG for IP (HRP) (ab131368) at 1/50000 dilution
  
  Predicted band size: 33 kDa
  
  
  Exposure time: 26 seconds
  
  
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] (ab11159)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/1000 (1.125 ug/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on the human cerebrum. The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Frozen sections) - Anti-Myelin Basic Protein antibody [MBP2] (ab11159)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10 (112.5 ug/ml) dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

  Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488).

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] (ab11159)

  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the rat cerebrum. The section was incubated with ab11159 for 30 mins at room temperature and blocked mouse IgG by specific antibody ab125913 for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] (ab11159)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Myelin Basic Protein with ab11159 at 1/10000 (0.113 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the mouse cerebrum. The section was incubated with ab11159 for 30 mins at room temperature and blocked mouse IgG by specific antibody ab125913 for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [MBP2] (ab11159)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myelin Basic Protein with ab11159 at 1:1000 (1.125 μg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control: No staining on human spleen (PMID: 9518636). The section was incubated with ab11159 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer).

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Anti-Myelin Basic Protein antibody [MBP2] (ab11159)