Anti-MDC1抗体
参阅全部 MDC1 一抗
兔多克隆抗体to MDC1
Rabbit
适用于: WB, IP, IHC-Pmore details
与反应: Human
预测可用于: Chimpanzee
Synthetic peptide corresponding to Human MDC1 aa 1375-1425.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab11171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/5000 - 1/25000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa). |
| IP | Use at 2-4 µg/mg of lysate. | |
| IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 9656 Human
GenBank: NP_055456 Human
Omim: 607593 Human
SwissProt: Q14676 Human
Unigene: 653495 Human
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Western blot - Anti-MDC1 antibody (ab11171)
All lanes : Anti-MDC1 antibody (ab11171) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 220 kDa
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody (ab11171)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MDC1 with ab11171 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-MDC1 antibody (ab11171)
1 mg of HeLa whole cell lysate was incubated with ab11171, 3 µg/mL. For western blotting ab11171, 1 µg/mL was used to confirm successful immunoprecipation.
