Anti-S100 alpha抗体
参阅全部 S100 alpha 一抗
兔多克隆抗体to S100 alpha
Rabbit
适用于: IHC-P, ICC/IFmore details
与反应: Human
Full length native protein (purified) corresponding to Human S100 alpha. Purified S100-alpha protein from human pectoral muscle cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 3% BSA
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab11428于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. | |
| ICC/IF | 1/50 - 1/200. |
Entrez Gene: 6271 Human
Omim: 176940 Human
SwissProt: P23297 Human
Unigene: 515715 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody (ab11428)
ab11428 (2µg/ml) staining S100 alpha in human breast tissue using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic compartment within the breast ductal regions
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody (ab11428)
ICC/IF image of ab11428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11428, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody (ab11428)
ab11428 labelling S100 alpha (red) in HEK293T cells by Immunocytochemistry/Immunofluorescence. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1:100 in blocking buffer) overnight at 4ºC. A fluorophore-conjugated goat anti-rabbit IgG (1:200) was used as the secondary antibody (1 hour at room temperature). Blue (DAPI) - nuclei. Images were taken at 40X magnification.
