Anti-Mad2L1 antibody [17D10]

• 产品名称

  Anti-Mad2L1抗体[17D10]
  参阅全部 Mad2L1 一抗

• 描述

  小鼠单克隆抗体[17D10] to Mad2L1

• 宿主

  Mouse

• 特异性

  Western blot with a variety of human cell lines confirms that the antibody is specific for Mad2.

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant human Mad2.

• 阳性对照

• WB: HAP1, HeLa and A431 cell lysates

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from supernatant.

• 克隆

  单克隆

• 克隆编号

  17D10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10691于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4085 Human

• Omim: 601467 Human

• SwissProt: Q13257 Human

• Unigene: 591697 Human

• 别名

• MAD 2A antibody

• MAD2 like 1 antibody

• MAD2 like protein 1 antibody

• MAD2 mitotic arrest deficient like 1 antibody

• MAD2-like protein 1 antibody

• Mad2l1 antibody

• MD2L1_HUMAN antibody

• Mitotic arrest deficient 2-like protein 1 antibody

• Mitotic arrest deficient yeast homolog like 1 antibody

• Mitotic spindle assembly checkpoint protein MAD2A antibody

• HsMAD 2 antibody

• HsMAD2 antibody

• MAD 2 antibody

• 

  Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)

  All lanes : Anti-Mad2L1 antibody [17D10] (ab10691) at 2 µg/ml
  
  Lane 1 : Wild-type HAP1 whole cell lysate
  Lane 2 : Mad2L1 knockout HAP1 whole cell lysate
  Lane 3 : HeLa whole cell lysate
  Lane 4 : A431 whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 24.6 kDa
  Observed band size: 24 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1 - 4: Merged signal (red and green). Green - ab10691 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

  ab10691 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab10691 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody [17D10] (ab10691)

  ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

• 

  Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)

  Western blot using ab10691 at 1/500.
  
  Lane 1: Hela Nuclear
  Lane 2: Hela
  Lane 3: A431
  Lane 4: Jurkat
  Lane 5: 293

  Mad2L1 predicted size: 24.6kD.

  Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
  Exposure time: 1min
  20µg of lysate per lane.

  Western blot using ab10691 at 1/500. Lane 1: Hela Nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293 Mad2L1 predicted size: 24.6kD. Secondary ab: anti-mouse HRP (ab6728) (1/5000) Exposure time: 1min 20µg of lysate per lane.

• 

  Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)This image is courtesy of an Abreview submitted by Saira Sarfraz

  Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.
  
  Secondary Antibody: An HRP-conjugated Goat anti-mouse polyclonal (1/100000).
  
  Blocking Step: 5% Milk for 16 hours at 4°C

  See Abreview