Anti-DMC1 antibody [2H12/4]

• 产品名称

  Anti-DMC1抗体[2H12/4]
  参阅全部 DMC1 一抗

• 描述

  小鼠单克隆抗体[2H12/4] to DMC1

• 宿主

  Mouse

• 特异性

  Specific for DMC1 - does not cross-react with the related protein Rad51. The antibody was tested on mouse meiotic chromosomes. It allowed localisation of the Dmc1 protein as numerous foci in the Leptotene and Zygotene stages of meiotic prophase, and fewer foci in the early Pachytene stage of meiotic prophase.

• 经测试应用

  适用于: IP, WB, IHC-P, Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  

• 免疫原

  The details of the immunogen for this antibody are not available.

• 阳性对照

• ICC:mouse meiotic chromosomes

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  2H12/4

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab11054于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 618398 Cow

• Entrez Gene: 11144 Human

• Entrez Gene: 13404 Mouse

• Entrez Gene: 362960 Rat

• Omim: 602721 Human

• SwissProt: Q14565 Human

• SwissProt: Q61880 Mouse

• Unigene: 339396 Human

• Unigene: 2524 Mouse

别名

• Disrupted meiotic cDNA 1 homolog antibody

• Disrupted meiotic cDNA 1, yeast, homolog of antibody

• dJ199H16.1 antibody

• DMC 1 antibody

• DMC1 antibody

• DMC1 dosage suppressor of mck1 homolog antibody

• DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody

• DMC1 homologue antibody

• DMC1_HUMAN antibody

• DMC1H antibody

• DNA meiotic recombinase 1 antibody

• HsLim15 antibody

• LIM15 antibody

• Meiotic recombination protein DMC1/LIM15 homolog antibody

• MGC150472 antibody

• MGC150473 antibody

Flow Cytometry - Anti-DMC1 antibody [2H12/4] (ab11054)

Overlay histogram showing HeLa cells stained with ab11054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.