Anti-hnRNP U/p120 antibody [3G6]

• 产品名称

  Anti-hnRNP U/p120抗体[3G6]
  参阅全部 hnRNP U/p120 一抗

• 描述

  小鼠单克隆抗体[3G6] to hnRNP U/p120

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human
  不与反应: Chicken, Lizard

• 免疫原

  Full length native protein (purified) corresponding to Human hnRNP U/p120. Crosslinked RNPs eluted from an oligo (dt) cellulose column.
  Database link: Q00839

• 阳性对照

• WB: HeLa cytoplasmic and nuclear fraction. HEK-293T whole cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human cerebellum tissue. Flow Cytometry: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Protein A purified from tissue culture supernatant.

• 克隆

  单克隆

• 克隆编号

  3G6

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10297于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3192 Human

• Omim: 602869 Human

• SwissProt: Q00839 Human

• Unigene: 106212 Human

• 别名

• HNRNPU antibody

• hnRNPU protein antibody

• HNRPU antibody

• HNRPU_HUMAN antibody

• p120 antibody

• p120 nuclear protein antibody

• pp120 antibody

• SAF A antibody

• SAF-A antibody

• SAFA antibody

• Scaffold attachment factor A antibody

• U21.1 antibody

• Heterogeneous nuclear ribonucleoprotein U antibody

• hnRNP U antibody

• hnRNP U protein antibody

• 

  Western blot - Anti-hnRNP U/p120 antibody [3G6] (ab10297)

  All lanes : Anti-hnRNP U/p120 antibody [3G6] (ab10297) at 1/1000 dilution
  
  Lane 1 : Cytoplasmic fraction of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
  Lane 2 : Nuclear fraction of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
  
  Predicted band size: 120 kDa
  Observed band size: 120 kDa
  
  
  
  This correlates with the information published in the reference Dreyfuss, G. et. al.

• 

  Western blot - Anti-hnRNP U/p120 antibody [3G6] (ab10297)This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov

  Anti-hnRNP U/p120 antibody [3G6] (ab10297) at 1/5000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 100 µg
  
  Secondary
  HRP conjugated sheep anti-mouse IgG
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 120 kDa
  Observed band size: 120 kDa
  Additional bands at: 60 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 1 minute
  

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [3G6] (ab10297)

  ab10297 (4 µg/ml)) staining hnRNP U/p120 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of Purkinje cells neurons.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H₂O₂ in methanol for 10 minutes. They were then blocked with a protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• 

  Flow Cytometry - Anti-hnRNP U/p120 antibody [3G6] (ab10297)

  Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab10297 (red line). The cells were fixed with 100% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10297, 1 µg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.