Anti-KAT13A / SRC1 antibody

• 产品名称

  Anti-KAT13A / SRC1抗体
  参阅全部 KAT13A / SRC1 一抗

• 描述

  兔多克隆抗体to KAT13A / SRC1

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IPmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant

• 免疫原

  Synthetic peptide within Human KAT13A/ SRC1 aa 1400-1500 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
  Database link: Q15788

• 阳性对照

• Nuclear extract from HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance of 1.4 at 280 nm equals 1.0 mg of IgG.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab10308于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 8648 Human

• Entrez Gene: 313929 Rat

• Omim: 602691 Human

• SwissProt: Q15788 Human

• Unigene: 596314 Human

• 别名

• Hin 2 protein antibody

• Hin2 protein antibody

• MGC129719 antibody

• MGC129720 antibody

• mNRC 1 antibody

• NCoA 1 antibody

• NCoA-1 antibody

• Ncoa1 antibody

• NCOA1_HUMAN antibody

• Nuclear receptor coactivator 1 antibody

• Nuclear receptor coactivator protein 1 antibody

• NY REN 52 antigen antibody

• Protein Hin 2 antibody

• Protein Hin-2 antibody

• Protein Hin2 antibody

• Renal carcinoma antigen NY REN 52 antibody

• Renal carcinoma antigen NY-REN-52 antibody

• RIP 160 antibody

• RIP160 antibody

• SRC 1 antibody

• SRC-1 antibody

• Steroid receptor coactivator 1 antibody

• bHLHe74 antibody

• Class E basic helix-loop-helix protein 74 antibody

• F SRC 1 antibody

• 

  Immunoprecipitation - Anti-KAT13A / SRC1 antibody (ab10308)

  Detection of KAT13A / SRC1 by western blot of immunoprecipitates.
  Samples: Lane 1 and 2: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells prepared using NETN lysis buffer. Lane 3: Control IgG.
  Antibodies: ab10308 (lot 2, lane 2) used for IP at 6 µg per reaction. KAT13A / SRC1 was also immunoprecipitated by a previous lot of this antibody (lot 1, lane 1). For blotting immunoprecipitated KAT13A / SRC1, ab10308 was used at 0.4 µg/mL.
  Detection: Chemiluminescence with an exposure time of 30 seconds.

• 

  Western blot - Anti-KAT13A / SRC1 antibody (ab10308)

  All lanes : Anti-KAT13A / SRC1 antibody (ab10308) at 0.1 µg/ml
  
  Lane 1 : HEK293T whole cell lysate at 50 µg
  Lane 2 : HEK293T whole cell lysate at 15 µg
  Lane 3 : HEK293T whole cell lysate at 5 µg
  Lane 4 : NIH/3T3 whole cell lysate at 50 µg
  
  Predicted band size: 156 kDa
  
  
  Exposure time: 30 seconds