Anti-CREBBP antibody

• 产品名称

  Anti-CREBBP抗体
  参阅全部 CREBBP 一抗

• 描述

  兔多克隆抗体to CREBBP

• 宿主

  Rabbit

• 经测试应用

  适用于: IP, IHC-P, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

• 免疫原

  Synthetic peptide corresponding to Human CREBBP aa 2392-2442.
  Database link: Q92793
  (Peptide available as ab4916)

• 阳性对照

• WB: HeLa whole cell lysate IHC-P: human prostate carcinoma tissue

• 常规说明

  Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.

  
  

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Antibodies were affinity purified using the peptide immobilized on solid support.

• Primary antibody说明

  Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab10490于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1387 Human

• Entrez Gene: 12914 Mouse

• Omim: 600140 Human

• SwissProt: Q92793 Human

• SwissProt: P45481 Mouse

• Unigene: 459759 Human

• Unigene: 132238 Mouse

• Unigene: 392384 Mouse

• 别名

• CREB-binding protein antibody

• Crebbp antibody

• Cyclic AMP responsive enhancer binding protein antibody

• KAT3A antibody

• RSTS antibody

• RTS antibody

• Rubinstein Taybi syndrome antibody

• CBP antibody

• CBP_HUMAN antibody

• CREB binding protein antibody

• 

  Western blot - Anti-CREBBP antibody - ChIP Grade (ab10490)

  All lanes : Anti-CREBBP antibody (ab10490) at 0.4 µg/ml
  
  Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  
  Predicted band size: 265 kDa
  
  
  Exposure time: 3 minutes
  
  
  

  Detected by chemiluminescence.

• 

  Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)

  ab10490 immunoprecipitating CREBBP at 6 µg/ml lysate.

  Lane 1: Anti-CREBBP antibody ab10489 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).

  Lane 2: Anti-CREBBP antibody ab10490 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).

  Lane 3: IgG control.

  For blotting immunoprecipitated CREBBP, ab10490 was used at 1 µg/mL.

  Detection: Chemiluminescence with an exposure time of 30 seconds.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CREBBP with ab10490 at 1/500 (2µg/ml). Detection: DAB.