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Human Resistin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Resistin in Human in Biofluids, Cell Culture Media samples.
隐藏别名
FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, RETN, Resistin, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, ADSF
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 2 pg/mL
1.4 - 400 pg/mL
Microplate
Abcam's Resistin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Resistin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100634.
Representative Standard Curve using ab100634.
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