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Human TGF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TGF alpha in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Protransforming growth factor alpha, TGFA
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 3 pg/mL
1.37 - 1000 pg/mL
Microplate
Abcam's TGF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF alpha in serum, plasma, cell culture supernatants
This assay employs an antibody specific for Human TGF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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-20°C
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Representative standard curve using ab100646
Representative standard curve using ab100646
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