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Human PDGF AA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PDGF AA in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CD306, LAIR2, Leukocyte-associated immunoglobulin-like receptor 2, LAIR-2
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 40 pg/mL
40 - 30000 pg/mL
Microplate
Abcam's PDGF AA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PDGF AA coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100622.
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