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Human CXCL7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CXCL7 in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.
隐藏别名
CTAP3, CXCL7, SCYB7, TGB1, THBGB1, PPBP, Platelet basic protein, PBP, C-X-C motif chemokine 7, Leukocyte-derived growth factor, Macrophage-derived growth factor, Small-inducible cytokine B7, LDGF, MDGF
Colorimetric
Plasma, Cell culture supernatant, Serum, Cell Lysate
Human
Sandwich (quantitative)
< 8.5 pg/mL
4.1 - 1000 pg/mL
Microplate
Abcam's CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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CXCL7 measured in biological fluids showing quantity (ng) per mL of tested sample
Representative Standard Curve using ab100613.
Representative Standard Curve using ab100613.
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