Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:2000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
ALK (D5F3) Rabbit Monoclonal Antibody detects endogenous levels of total ALK protein as well as ALK fusion proteins, such as EML4-ALK variants and NPM-ALK, even at low levels. This antibody does not cross-react with other family members.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues in the carboxy terminus of human ALK.
