| 存储条件 |
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| Product Name | SMC1 [SN20-27] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDLS2 antibody DKFZp686L19178 antibody DXhXs423e antibody DXS423E antibody KIAA0178 antibody MGC138332 antibody OTTHUMP00000061876 antibody RP6 29D12.1 antibody SB1.8 antibody Segregation of mitotic chromosomes 1 antibody Segregation of mitotic chromosomes like 1 antibody SMC 1 antibody SMC protein 1B antibody SMC-1-beta antibody SMC-1B antibody SMC1 antibody SMC1A antibody SMC1alpha antibody SMC1alpha protein antibody SMC1B antibody SMC1B_HUMAN antibody SMC1BETA antibody SMC1beta protein antibody SMC1L1 antibody SMC1L2 antibody SMCB antibody Structural maintenance of chromosome 1 like 1 protein antibody Structural maintenance of chromosome 1 like 2 protein antibody Structural maintenance of chromosomes 1A antibody Structural maintenance of chromosomes 1B antibody Structural maintenance of chromosomes protein 1B antibody |
| Product description | The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. SMC1α (structural maintenance of chromosomes protein 1A), also known as SMC1, SMCB, CDLS2, SB1.8, SMC1L1 or DXS423E, is a 1,233 amino acid nuclear protein that is involved in chromosome cohesion during the cell cycle. SMC1α interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role in DNA repair. SMC1α is a component of the cohesion complex, which is required for the cohesion of sister chromatids after DNA replication. Mutations in the gene encoding SMC1α may be the cause of Cornelia de Lange syndrome (CdLS), which is a clinically heterogeneous developmental disorder characterized by facial dysmorphia, upper limb malformations, growth and cognitive retardation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CDLS2 antibody DKFZp686L19178 antibody DXhXs423e antibody DXS423E antibody KIAA0178 antibody MGC138332 antibody OTTHUMP00000061876 antibody RP6 29D12.1 antibody SB1.8 antibody Segregation of mitotic chromosomes 1 antibody Segregation of mitotic chromosomes like 1 antibody SMC 1 antibody SMC protein 1B antibody SMC-1-beta antibody SMC-1B antibody SMC1 antibody SMC1A antibody SMC1alpha antibody SMC1alpha protein antibody SMC1B antibody SMC1B_HUMAN antibody SMC1BETA antibody SMC1beta protein antibody SMC1L1 antibody SMC1L2 antibody SMCB antibody Structural maintenance of chromosome 1 like 1 protein antibody Structural maintenance of chromosome 1 like 2 protein antibody Structural maintenance of chromosomes 1A antibody Structural maintenance of chromosomes 1B antibody Structural maintenance of chromosomes protein 1B antibody |
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| Molecular Weight(MW) | 143 kDa |
| Cellular Localization | Nucleus, Chromosome. |
Application
Fig1: Western blot analysis of SMC1 on different lysates using anti-SMC1 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Hela
Application
Fig2: ICC staining SMC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining SMC1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining SMC1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SMC1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC1 antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, A431, Hela, SW480, human tonsil tissue, mouse colon tissue, human breast carcinoma tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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