2610318I18Rik antibody kelch-like 22 antibody kelch-like family member 22 antibody Kelch-like protein 22 antibody KELCHL antibody KLH22_HUMAN antibody Klhl22 antibody RGD1306288 antibody
Product description
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.
Immunogen
Recombinant protein
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBICC
WB:1:500 ICC: 1:100-1:500
Species Reactivity
HumanMouse
Concentration
2mg/ml
Target Information
Alternative Names
2610318I18Rik antibody kelch-like 22 antibody kelch-like family member 22 antibody Kelch-like protein 22 antibody KELCHL antibody KLH22_HUMAN antibody Klhl22 antibody RGD1306288 antibody
Fig1: Western blot analysis of KLHL22 on mouse brain tissue lysate using anti-KLHL22 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining KLHL22 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Positive Control
Mouse brain tissue lysate, U251.
Application Notes
WB:1:500 ICC: 1:100-1:500
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
