Pax genes contain paired domains that share strong homology to genes in Drosophila which are involved in programming early development. The product of the Pax-3 gene is a DNA-binding protein expressed during early neurogenesis. Pax-3 is a protein containing both a paired domain and a paired-type homeodomain. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme. Mutations in the MITF and Pax-3 genes, encoding transcriptions factors, are responsible for Waardenburg syndrome II (WS2) and WS1/WS3, respectively. Pax-7 is a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax-7 is also expressed in satellite cells residing in adult muscle. The gene which encodes Pax-7 maps to human chromosome 1p36.2-p36.12.
Fig1: Western blot analysis of PAX3 on human PAX3 recombinant protein using anti-PAX3 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PAX3 on HEK293 (1) and PAX3-hIgGFc transfected HEK293 (2) cell lysate using anti-PAX3 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of PAX3 on different cell lysate using anti-PAX3 antibody at 1/1,000 dilution. Positive control：Line1:Mouse brainLine2:Rat spleenLine3:Mouse liver
Positive Control
Human PAX3 recombinant protein, PAX3-hIgGFc transfected HEK293 cell lysate, Mouse brain, Rat spleen, Mouse liver cell lysate.
Application Notes
WB:1:500-1:1,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
