| 存储条件 |
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| Product Name | SIRT7 [B2-E2] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | NAD dependent deacetylase sirtuin 7 antibody NAD dependent protein deacetylase sirtuin 7 antibody NAD-dependent protein deacetylase sirtuin-7 antibody Regulatory protein SIR2 homolog 7 antibody Silent mating type information regulation 2 homolog antibody Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody SIR2 L7 antibody SIR2 like protein 7 antibody Sir2 related protein type 7 antibody SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody SIR2-like protein 7 antibody SIR2L 7 antibody SIR2L7 antibody SIR7_HUMAN antibody SIRT 7 antibody SIRT7 antibody Sirtuin 7 antibody Sirtuin type 7 antibody Sirtuin7 antibody |
| Product description | Sirtuins (SIRT1-7) are human homologs of the yeast Sir2 (silent information regulator-2) protein and are divided into four main classes: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. In S. cerevisiae, Sir2 deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA (ribosomal DNA) and silent mating-type loci. The human SIRT proteins are NAD-dependent deacetylases that act as intracellular regulators and are thought to have ribosyltransferase activity. SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer. Two isoforms exist due to alternative splicing events. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | NAD dependent deacetylase sirtuin 7 antibody NAD dependent protein deacetylase sirtuin 7 antibody NAD-dependent protein deacetylase sirtuin-7 antibody Regulatory protein SIR2 homolog 7 antibody Silent mating type information regulation 2 homolog antibody Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody SIR2 L7 antibody SIR2 like protein 7 antibody Sir2 related protein type 7 antibody SIR2 S.CEREVISIAE HOMOLOG-LIKE 7 antibody SIR2-like protein 7 antibody SIR2L 7 antibody SIR2L7 antibody SIR7_HUMAN antibody SIRT 7 antibody SIRT7 antibody Sirtuin 7 antibody Sirtuin type 7 antibody Sirtuin7 antibody |
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| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm. Nucleus, nucleolus. |
| SwissProt ID | Q9NRC8 |
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Application
Fig1: Western blot analysis of SIRT7 on human SIRT7 recombinant protein using anti-SIRT7 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of SIRT7 on HEK293 (1) and SIRT7-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT7 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with SIRT7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human SIRT7 recombinant protein, SIRT7-hIgGFc transfected HEK293 cell lysate, Hela. |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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