JMJD2B (JmjC domain-containing histone demethylation protein 3A) is a 1,064 amino acid protein encoded by the human gene JMJD2B. JMJD2B belongs to the JMJD2B histone demethylase family and contains one JmjC domain, one JmjN domain, two PHD-type zinc fingers and two Tudor domains. The two Tudor domains recognize and bind methylated histones and have an interdigitated structure; the unusual fold is required for its ability to bind methylated histone tails. JMJD2B is a histone demethylase that specifically demethylates Lys 9 residues of Histone H3, thereby playing a role in histone code. It does not demethylate Histone H3 Lys 4, H3 Lys 27, H3 Lys 36 or H4 Lys 20, however, and is only able to demethylate trimethylated H3 Lys-9 and has weaker activity than JMJD2A, JMJD2C and JMJD2D. JMJD2B demethyl-ation of Lysine residues will generate formaldehyde and succinate. JMJD2B is a ubiquitously expressed nuclear protein.
Fig1: Western blot analysis of KDM4B on human KDM4B recombinant protein using anti-KDM4B antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of KDM4B on HEK293 (1) and KDM4B-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM4B antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of NIH/3T3 cells with KDM4B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human KDM4B recombinant protein, KDM4B-hIgGFc transfected HEK293 cell lysate, NIH/3T3.
Application Notes
WB:1:500-1:2,000 FC:1:100-1:200
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
