| 存储条件 |
|---|
| Product Name | ANAPC10 [D10-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANAPC 10 antibody anapc10 antibody Anaphase promoting complex 10 antibody Anaphase promoting complex subunit 10 antibody Anaphase-promoting complex subunit 10 antibody Apc 10 antibody APC10 antibody APC10_HUMAN antibody Cyclosome subunit 10 antibody DKFZP564L0562 antibody Doc 1 antibody Doc1 antibody Doc1, S. cerevisiae, homolog of antibody OTTHUMP00000220297 antibody OTTHUMP00000220298 antibody OTTHUMP00000220299 antibody OTTHUMP00000220300 antibody OTTHUMP00000220303 antibody |
| Product description | Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ANAPC 10 antibody anapc10 antibody Anaphase promoting complex 10 antibody Anaphase promoting complex subunit 10 antibody Anaphase-promoting complex subunit 10 antibody Apc 10 antibody APC10 antibody APC10_HUMAN antibody Cyclosome subunit 10 antibody DKFZP564L0562 antibody Doc 1 antibody Doc1 antibody Doc1 S. cerevisiae homolog of antibody OTTHUMP00000220297 antibody OTTHUMP00000220298 antibody OTTHUMP00000220299 antibody OTTHUMP00000220300 antibody OTTHUMP00000220303 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Anaphase-promoting complex, cytosol, nucleoplasm |
| SwissProt ID | Q9UM13 |
|---|
Application
Fig1: Western blot analysis of ANAPC10 on human ANAPC10 recombinant protein using anti-ANAPC10 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of ANAPC10 on HEK293 (1) and ANAPC10-hIgGFc transfected HEK293 (2) cell lysate using anti-ANAPC10 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of ANAPC10 on different cell lysate using anti-ANAPC10 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: MCF-7 Line3: SK-Br-3 Line4: A431 Line5: HEK293 Line6: A549 Line7: SPC-A-1
Application
Fig4: ICC staining ANAPC10 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ANAPC10 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-ANAPC10 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Hela cells with ANAPC10 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human ANAPC10 recombinant protein, ANAPC10-hIgGFc transfected HEK293 cell lysate, Hela, MCF-7, SK-Br-3, A431, HEK293, A549, SPC-A-1 cell lysate, human colon cancer tissue, human esophageal cancer tissue, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
