Hyaluronic acid (HA) is a nonsulfated glycosaminoglycan that regulates cell adhesion and migration. HA effects are mediated through two receptors, CD44 (also designated HCAM) and the receptor of hyaluronic acid mediated motility (RHAMM). RHAMM, also designated intracellular hyaluronic acid binding protein (IHABP) and CD168, is a matrix receptor, which is linked to the plasma membrane by a GPI anchor and regulates cell motility. RHAMM expression is upregulated in malignant lymphoid tissues and is subsequently implicated in tumor progression and metastasis formation, as well as signal transduction. Although still unclear, RHAMM is thought to exist as several isoforms ranging in size. A variant isoform, designated v4, is a protein that when over-expressed, is thought to be the cause of transformation and metastasis formation in fibroblasts
Fig1: Western blot analysis of CD168 on human CD168 recombinant protein using anti-CD168 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CD168 on HEK293 (1) and CD168-hIgGFc transfected HEK293 (2) cell lysate using anti-CD168 antibody at 1/1,000 dilution.
Application
Fig3: Flow cytometric analysis of Hela cells with CD168 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Positive Control
Human CD168 recombinant protein, CD168-hIgGFc transfected HEK293 cell lysate, Hela
Application Notes
WB:1:500-1:2,000 FC:1:50-1:100
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
