| 存储条件 |
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| Product Name | UHRF1 [B1-A1] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | Ac2-121 antibody AL022808 antibody E3 ubiquitin-protein ligase UHRF1 antibody EC 6.3.2.- antibody FLJ21925 antibody hNP95 antibody hUHRF1 antibody HuNp95 antibody ICBP90 antibody Inverted CCAAT box binding protein of 90 kDa antibody Inverted CCAAT box binding protein, 90-kD antibody Inverted CCAAT box-binding protein of 90 kDa antibody Liver regeneration-related protein LRRG126 antibody MGC138707 antibody NP95 antibody Nuclear phosphoprotein 95 antibody Nuclear phosphoprotein, 95-KD antibody Nuclear protein 95 antibody Nuclear zinc finger protein Np95 antibody RING finger protein 106 antibody RNF106 antibody TDRD22 antibody Transcription factor ICBP90 antibody Ubiquitin like containing PHD and RING finger domains protein 1 antibody Ubiquitin like PHD and RING finger domain containing protein 1 antibody Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody Ubiquitin-like protein containing PHD and RING finger domains 1 antibody Ubiquitin-like with PHD and ring finger domains 1 antibody Ubiquitin-like, containing PHD and RING finger domains, 1 antibody Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody UHRF1 antibody UHRF1_HUMAN antibody |
| Product description | UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1), also known as Np95 (nuclear zinc finger protein 95), ICBP90 (inverted CCAAT box-binding protein of 90 kDa) or RNF106, is a transcription and cell cycle regulator belonging to the RING-finger type E3 ubiquitin ligase subfamily. UHRF1 is expressed in bone marrow, thymus, heart, testis and lung, and contains one PHD-type zinc finger, a ubiquitin-like domain, two RING-type zinc fingers and one YDG/SRA domain. Localizing to the nucleus, UHRF1 is believed to function as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation. By mediating ubiquitination, UHRF1 plays an important role in cellular proliferation. In addition, UHRF1 directly interacts with Dnmt1 (a maintenance DNA methyltransferase) and is required for the stable association of Dnmt1 with chromatin. UHRF1 is overexpressed in cancer cells, suggesting a possible role in carcinogenesis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
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| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Ac2-121 antibody AL022808 antibody E3 ubiquitin-protein ligase UHRF1 antibody EC 6.3.2.- antibody FLJ21925 antibody hNP95 antibody hUHRF1 antibody HuNp95 antibody ICBP90 antibody Inverted CCAAT box binding protein of 90 kDa antibody Inverted CCAAT box binding protein 90-kD antibody Inverted CCAAT box-binding protein of 90 kDa antibody Liver regeneration-related protein LRRG126 antibody MGC138707 antibody NP95 antibody Nuclear phosphoprotein 95 antibody Nuclear phosphoprotein 95-KD antibody Nuclear protein 95 antibody Nuclear zinc finger protein Np95 antibody RING finger protein 106 antibody RNF106 antibody TDRD22 antibody Transcription factor ICBP90 antibody Ubiquitin like containing PHD and RING finger domains protein 1 antibody Ubiquitin like PHD and RING finger domain containing protein 1 antibody Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody Ubiquitin-like protein containing PHD and RING finger domains 1 antibody Ubiquitin-like with PHD and ring finger domains 1 antibody Ubiquitin-like containing PHD and RING finger domains 1 antibody Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody UHRF1 antibody UHRF1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 90 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q96T88 |
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Application
Fig1: Western blot analysis of UHRF1 on human UHRF1 recombinant protein using anti-UHRF1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of UHRF1 on HEK293 (1) and UHRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-UHRF1 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of UHRF1 on MCF-7 (1) and Hela (2) cell lysate using anti-UHRF1 antibody at 1/1,000 dilution.
Application
Fig4: ICC staining UHRF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-UHRF1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using anti-UHRF1 antibody. Counter stained with hematoxylin.
Application
Fig7:Flow cytometric analysis of MCF-7 cells with UHRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human UHRF1 recombinant protein, UHRF1-hIgGFc transfected HEK293 cell lysate, MCF-7, Hela, human bladder cancer tissues, human esophageal cancer tissues. |
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| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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