For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
Application
Dilution
Western Blotting
1:1000
Immunoprecipitation
1:25
Immunohistochemistry (Paraffin)
1:800
Immunofluorescence (Immunocytochemistry)
1:400
Flow Cytometry (Fixed/Permeabilized)
1:200
Chromatin IP
1:50
Chromatin IP-seq
1:50
CUT&RUN
1:50
CUT&Tag
1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #96075.
Specificity / Sensitivity
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit Monoclonal Antibody detects endogenous levels of histone H3 only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones.
Species Reactivity:
Human, Mouse, Rat, Monkey, Zebrafish
Species predicted to react based on 100% sequence homology:
S. cerevisiae
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated.
