Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
p38 MAPK Antibody detects endogenous levels of total p38α, -β or -γ MAPK protein. This antibody does not recognize p38δ, JNK/SAPK or p44/42 MAPK.
Species Reactivity:
Human, Mouse, Rat, Monkey, Guinea Pig
Species predicted to react based on 100% sequence homology:
Chicken
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro350 of human p38 MAPK protein. Antibodies are purified by protein A and peptide affinity chromatography.
