Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|
| Western Blotting | 1:2000 |
| Fluorescent Western | 1:2000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:3200 |
| Chromatin IP | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #61121.
Specificity / Sensitivity
Rb (4H1) Mouse mAb detects endogenous levels of total Rb protein. The antibody does not cross-react with the Rb homologues p107 or p130, or with other proteins.
Species Reactivity:
Human, Monkey, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a Rb-C fusion protein containing residues 701-928 of human Rb. The epitope corresponds to a region surrounding His890 of human Rb.
