For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application
Dilution
Western Blotting
1:1000
Immunoprecipitation
1:100
Immunofluorescence (Immunocytochemistry)
1:400 - 1:800
Flow Cytometry (Fixed/Permeabilized)
1:100 - 1:400
Chromatin IP
1:100
Chromatin IP-seq
1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #55607.
Specificity / Sensitivity
SMAD2/3 (D7G7) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMAD2/3 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His198 of human SMAD2/3 protein.
