Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
LC3B Antibody detects endogenous levels of total LC3B protein. Cross-reactivity may exist with other LC3 isoforms. Stronger reactivity is observed with the type II form of LC3B.
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology:
Monkey, Bovine, Pig
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LC3B. Antibodies were purified by peptide affinity chromatography.
