For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
Application
Dilution
Western Blotting
1:1000
Simple Western™
1:10 - 1:50
Immunoprecipitation
1:300
IHC Leica Bond
1:50 - 1:200
Immunohistochemistry (Paraffin)
1:50
Immunofluorescence (Frozen)
1:100 - 1:400
Immunofluorescence (Immunocytochemistry)
1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)
1:100 - 1:400
Chromatin IP
1:100
Chromatin IP-seq
1:100
CUT&RUN
1:100
CUT&Tag
1:100
eCLIP
1:200
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #16098.
Specificity / Sensitivity
Ezh2 (D2C9) Rabbit Monoclonal Antibody detects endogenous levels of total Ezh2 protein. This antibody does not cross-react with Ezh1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein.
