| 存储条件 |
|---|
| Product Name | CXCR4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human CXCR4 aa 253-352 / 352. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:1000 ICC/IF:1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCR4 |
|---|---|
| Gene Synonyms | FB22 HM89 LAP3 LCR1 NPYR WHIM CD184 LAP-3 LESTR NPY3R NPYRL WHIMS HSY3RR NPYY3R WHIMS1 D2S201E |
| Gene Full Name | C-X-C motif chemokine receptor 4 |
| Gene Summary | This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | C-X-C chemokine receptor type 4 antibody CD184 antibody CD184 antigen antibody Chemokine (C X C motif) receptor 4 antibody Chemokine CXC Motif Receptor 4 antibody CXC-R4 antibody CXCR-4 antibody CXCR4 antibody CXCR4_HUMAN antibody D2S201E antibody C-X-C chemokine receptor type 4 antibody CD184 antibody CD184 antigen antibody Chemokine (C X C motif) receptor 4 antibody Chemokine CXC Motif Receptor 4 antibody CXC-R4 antibody CXCR-4 antibody CXCR4 antibody CXCR4_HUMAN antibody D2S201E antibody FB22 antibody Fusin antibody HM89 antibody HSY3RR antibody LAP 3 antibody LAP3 antibody LCR1 antibody LESTR antibody Leukocyte derived seven transmembrane domain receptor antibody Leukocyte-derived seven transmembrane domain receptor antibody Lipopolysaccharide associated protein 3 antibody Neuropeptide Y receptor Y3 antibody NPY3R antibody NPYR antibody NPYRL antibody NPYY3 antibody NPYY3R antibody Probable G protein coupled receptor lcr1 homolog antibody SDF 1 receptor antibody SDF-1 receptor antibody SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody Stromal cell derived factor 1 receptor antibody Stromal cell-derived factor 1 receptor antibody WHIM antibody WHIMS antibody |
| Molecular Weight(MW) | 40kDa(Observed band size: 45kDa) |
| Cellular Localization | Cell membrane, Cell junction, Early endosome, Late endosome, Lysosome. |
WB
Western blot analysis of CXCR4 on different lysates with Rabbit anti-CXCR4 antibody at 1/1,000 dilution. Lane 1: Jurkat cell lysate (hot lysis), Lane 2: HeLa cell lysate (hot lysis), Lysates/proteins at 20 µg/Lane. Exposure time: 25 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CXCR4 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CXCR4 with Rabbit anti-CXCR4 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCR4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
