| 存储条件 |
|---|
| Product Name | FADS1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within N-terminal Human FADS1. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:100 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FADS1 |
|---|---|
| Gene Synonyms | D5D TU12 FADS6 FADSD5 LLCDL1 |
| Gene Full Name | fatty acid desaturase 1 |
| Gene Summary | The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008] |
| Alternative Names | D5D antibody Delta 5 desaturase antibody Delta 5 fatty acid desaturase antibody Delta(5) desaturase antibody Delta(5) fatty acid desaturase antibody Delta-5 desaturase antibody Fads1 antibody FADS1_HUMAN antibody FADS6 antibody FADSD5 antibody D5D antibody Delta 5 desaturase antibody Delta 5 fatty acid desaturase antibody Delta(5) desaturase antibody Delta(5) fatty acid desaturase antibody Delta-5 desaturase antibody Fads1 antibody FADS1_HUMAN antibody FADS6 antibody FADSD5 antibody Fatty acid desaturase 1 antibody FLJ38956 antibody Linoleoyl CoA desaturase (delta 6 desaturase) like 1 antibody LLCDL1 antibody TU12 antibody |
| Molecular Weight(MW) | 52kDa(Observed band size: 55kDa) |
| Cellular Localization | Mitochondrion, Endoplasmic reticulum membrane. |
WB
Western blot analysis of FADS1 on different lysates with Rabbit anti-FADS1 antibody at 1/2,000 dilution. Lane 1: Mouse liver tissue lysate, Lane 2: Rat liver tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FADS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling FADS1 with Rabbit anti-FADS1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FADS1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of FADS1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:100 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
