Human peripheral mononuclear cells enriched for T-cells
Key Feature
Clonality
monoclonal
Isotype
IgG2b,k
Host Species
Rat
Tested Applications
WB
WB:1.5ug/ml
Species Reactivity
HumanMonkeyMouse
Purification
Affinity purified
Target Information
Gene Symbol
CD52
Gene Synonyms
B7
Gene Full Name
CD52 molecule
Gene Summary
Predicted to be involved in positive regulation of cytosolic calcium ion concentration. Predicted to act upstream of or within response to bacterium. Predicted to be located in plasma membrane and side of membrane. Predicted to be active in sperm midpiece. Orthologous to human CD52 (CD52 molecule). [provided by Alliance of Genome Resources, Feb 2025]
Alternative Names
CDw52 HE5
Molecular Weight(MW)
11/25kDa
Cellular Localization
Cell membrane.
Application
WB
Western Blot using anti-CD52 antibody. Mouse spleen samples were resolved on a 15% SDS PAGE gel and blots probed with the rat IgG1-chimeric version of anti-CD52 antibody at 1.5 µg/ml before detection using an anti-rat secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD52 is ~8kDa, but this protein is extensively glycosylated and exhibits running sizes of ~11-25kDa. anti-CD52 antibody successfully detected CD52 in mouse spleen samples.
Application Notes
WB:1.5ug/ml
Additional Information
Form
Liquid
Storage Instructions
Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.