| 存储条件 |
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| Product Name | alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within N-terminal human alpha smooth muscle Actin. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:5000-1:50000 IHC:1:50000 ICC:1:2000 IF-P:1:1000 FC:1:1000-1:2000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACTA2 |
|---|---|
| Gene Synonyms | ACTSA SMDYS α-SMA |
| Gene Full Name | actin alpha 2, smooth muscle |
| Gene Summary | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017] |
| Alternative Names | alpha SMA a-SMA antibody asma antibody a actin antibody AAT6 antibody ACTA_HUMAN antibody ACTA2 antibody Actin alpha 2 smooth muscle aorta antibody Actin aortic smooth muscle antibody Actin aortic smooth muscle antibody alpha SMA a-SMA antibody asma antibody a actin antibody AAT6 antibody ACTA_HUMAN antibody ACTA2 antibody Actin alpha 2 smooth muscle aorta antibody Actin aortic smooth muscle antibody Actin aortic smooth muscle antibody ACTSA antibody ACTVS antibody Alpha 2 actin antibody Alpha actin 2 antibody Alpha cardiac actin antibody Alpha-actin-2 antibody Cell growth inhibiting gene 46 protein antibody Cell growth-inhibiting gene 46 protein antibody GIG46 antibody Growth inhibiting gene 46 antibody MYMY5 antibody |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of alpha smooth muscle Actin on different lysates with Rabbit anti-alpha smooth muscle Actin antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: A431 cell lysate, Lane 3: A549 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: C2C12 cell lysate, Lane 6: L6 cell lysate, Lane 7: Mouse heart tissue lysate, Lane 8: Mouse skin tissue lysate, Lane 9: Rat heart tissue lysate, Lane 10: Rat skin tissue lysate, Lane 11: Rat smooth muscle tissue lysate. Lysates/proteins at 20 µg/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-alpha smooth muscle Actin antibody at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling alpha smooth muscle Actin with Rabbit anti-alpha smooth muscle Actin antibody at 1/2,500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha smooth muscle Actin antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling alpha smooth muscle Actin. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody alpha smooth muscle Actin (red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 647 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
FC
Flow cytometric analysis of HepG2 cells labeling alpha smooth muscle Actin. Cells were fixed and permeabilized. Then stained with the primary antibody (1/2,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:5000-1:50000 IHC:1:50000 ICC:1:2000 IF-P:1:1000 FC:1:1000-1:2000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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