| 存储条件 |
|---|
| Product Name | Rabbit polyclonal antibody to UTRN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Utrophin/UTRN recombinant protein (Position: L1211-K2294). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC:1:50-1:400 | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UTRN |
|---|---|
| Gene Synonyms | DRP DMDL DRP1 |
| Gene Full Name | utrophin |
| Gene Summary | This gene shares both structural and functional similarities with the dystrophin gene. It contains an actin-binding N-terminus, a triple coiled-coil repeat central region, and a C-terminus that consists of protein-protein interaction motifs which interact with dystroglycan protein components. The protein encoded by this gene is located at the neuromuscular synapse and myotendinous junctions, where it participates in post-synaptic membrane maintenance and acetylcholine receptor clustering. Mouse studies suggest that this gene may serve as a functional substitute for the dystrophin gene and therefore, may serve as a potential therapeutic alternative to muscular dystrophy which is caused by mutations in the dystrophin gene. Alternative splicing of the utrophin gene has been described; however, the full-length nature of these variants has not yet been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | DMDL DRP DRP 1 DRP1 Dystrophin related protein 1 UTRN utrophin |
| Molecular Weight(MW) | 394kDa |
| Cellular Localization | Postsynaptic cell membrane,Cytoplasm, cytoskeleton. |
WB
Western blot analysis of UTRN using anti-UTRN antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UTRN antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
IF analysis of UTRN using anti-UTRN antibody. UTRN was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-UTRN Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).| Application Notes | WB:1:500-1:2000 ICC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
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