| 存储条件 |
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| Product Name | Nkx2.2 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Nkx2.2 aa 1-273. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIHCWB |
| WB:1:1000 IHC:1:200-1:1000 ICC:1:100 IF-F:1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NKX2-2 |
|---|---|
| Gene Synonyms | NKX2B NKX2.2 |
| Gene Full Name | NK2 homeobox 2 |
| Gene Summary | The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq, Jul 2008] |
| Alternative Names | Homeobox protein NK 2 homolog B antibody Homeobox protein NK-2 homolog B antibody Homeobox protein Nkx 2.2 antibody Homeobox protein Nkx-2.2 antibody NK 2 homolog B antibody NK2 homeobox 2 antibody NK2 transcription factor like protein B antibody NK2 transcription factor related locus 2 antibody NKX2 2 antibody NKX2-2 antibody Homeobox protein NK 2 homolog B antibody Homeobox protein NK-2 homolog B antibody Homeobox protein Nkx 2.2 antibody Homeobox protein Nkx-2.2 antibody NK 2 homolog B antibody NK2 homeobox 2 antibody NK2 transcription factor like protein B antibody NK2 transcription factor related locus 2 antibody NKX2 2 antibody NKX2-2 antibody NKX22 antibody NKX22_HUMAN antibody Nkx2b antibody tinman antibody |
| Molecular Weight(MW) | 30kDa(Observed band size: 34kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of Nkx2.2 on different lysates with Rabbit anti-Nkx2.2 antibody at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane) Lane 2: C6 cell lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Nkx2.2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of C6 cells labeling Nkx2.2 with Rabbit anti-Nkx2.2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nkx2.2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Immunofluorescence analysis of frozen mouse E14.5 embyro tissue with Rabbit anti-Nkx2.2 antibody at 1/500 dilution. Important Notice: Antigen retrieval is not required before IHC-Fr staining. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC:1:100 IF-F:1:500 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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