| 存储条件 |
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| Product Name | CGRP Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within rat CGRP aa 1-128. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-FIHCWB |
| WB:1:2000 IHC:1:200-1:1000 ICC:1:100 IF-F:1:500 FC:1:10000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CALCA |
|---|---|
| Gene Synonyms | CT KC PCT CGRP CALC1 CGRP1 CGRP-I CGRP-alpha |
| Gene Full Name | calcitonin related polypeptide alpha |
| Gene Summary | This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014] |
| Alternative Names | Calcitonin/calcitonin related polypeptide alpha antibody Alpha type CGRP antibody Beta type CGRP antibody CALC1 antibody CALC2 antibody CALCA antibody CALCB antibody Calcitonin 1 antibody Calcitonin 2 antibody Calcitonin gene related peptide I antibody Calcitonin/calcitonin related polypeptide alpha antibody Alpha type CGRP antibody Beta type CGRP antibody CALC1 antibody CALC2 antibody CALCA antibody CALCB antibody Calcitonin 1 antibody Calcitonin 2 antibody Calcitonin gene related peptide I antibody Calcitonin gene related peptide II antibody Calcitonin related polypeptide alpha antibody Calcitonin related polypeptide beta antibody CGRP antibody CGRP I antibody CGRP II antibody CGRP1 antibody CGRP2 antibody CT antibody Katacalcin antibody KC antibody |
| Molecular Weight(MW) | 14kDa(Observed band size: 10kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of CGRP on different lysates with Rabbit anti-CGRP antibody at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-CGRP antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of TT (positive) and HeLa (negative) labeling CGRP with Rabbit anti-CGRP antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CGRP antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Immunofluorescence analysis of frozen rat colon tissue with Rabbit anti-CGRP antibody at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody ( green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
FC
Flow cytometric analysis of HeLa (left, negative) and TT (right, positive) cells labeling CGRP. Cells were fixed and permeabilized. Then stained with the primary antibody (1/10,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:200-1:1000 ICC:1:100 IF-F:1:500 FC:1:10000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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