| 存储条件 |
|---|
| Product Name | HE4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human HE4 aa 78-114 / 124. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WFDC2 |
|---|---|
| Gene Synonyms | HE4 BENP WAP5 EDDM4 dJ461P17.6 |
| Gene Full Name | WAP four-disulfide core domain 2 |
| Gene Summary | This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008] |
| Alternative Names | dJ461P17.6 antibody EDDM4 antibody epididymal protein 4 antibody Epididymal secretory protein E4 antibody Epididymis specific whey acidic protein type four disulfide core antibody HE 4 antibody Major epididymis specific protein E4 antibody Major epididymis-specific protein E4 antibody MGC57529 antibody Putative protease inhibitor WAP5 antibody dJ461P17.6 antibody EDDM4 antibody epididymal protein 4 antibody Epididymal secretory protein E4 antibody Epididymis specific whey acidic protein type four disulfide core antibody HE 4 antibody Major epididymis specific protein E4 antibody Major epididymis-specific protein E4 antibody MGC57529 antibody Putative protease inhibitor WAP5 antibody WAP 5 antibody WAP domain containing protein HE4 antibody WAP domain containing protein HE4 V4 antibody WAP four disulfide core domain 2 antibody WAP four disulfide core domain protein 2 antibody WAP four-disulfide core domain protein 2 antibody WAP5 antibody WFDC 2 antibody WFDC2 antibody WFDC2_HUMAN antibody |
| Molecular Weight(MW) | 13kDa(Observed band size: 20kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of HE4 on different lysates with Rabbit anti-HE4 antibody at 1/2,000 dilution. Lane 1: SW480 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: OVCAR-3 cell lysate (15 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HepG2 cells labeling HE4 with Rabbit anti-HE4 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HE4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HepG2 cells labeling HE4. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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