| 存储条件 |
|---|
| Product Name | Liver Carboxylesterase 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Liver Carboxylesterase 1 aa 51-100 / 567. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 IHC:1:1000 ICC:1:100 FC:1:500-1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CES1 |
|---|---|
| Gene Synonyms | CEH REH TGH ACAT CE-1 CES2 HMSE SES1 HMSE1 PCE-1 hCE-1 |
| Gene Full Name | carboxylesterase 1 |
| Gene Summary | This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Alternative Names | ACAT antibody Acyl coenzyme A cholesterol acyltransferase antibody Acyl-coenzyme A:cholesterol acyltransferase antibody Brain carboxylesterase hBr1 antibody Carboxyesterase ES-3 antibody Carboxylesterase antibody Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody Carboxylesterase 1 antibody Carboxylesterase 1 deficiency included antibody Carboxylesterase 2 formerly antibody ACAT antibody Acyl coenzyme A cholesterol acyltransferase antibody Acyl-coenzyme A:cholesterol acyltransferase antibody Brain carboxylesterase hBr1 antibody Carboxyesterase ES-3 antibody Carboxylesterase antibody Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody Carboxylesterase 1 antibody Carboxylesterase 1 deficiency included antibody Carboxylesterase 2 formerly antibody CE 1 antibody CEH antibody Ces-1 antibody CES1 antibody CES2 antibody CESDD1 antibody Cholesterol ester hydrolase neutral macrophage-derived antibody Cholesteryl ester hydrolase antibody Cocaine carboxylesterase antibody EC 3.1.1.1 antibody Egasyn antibody ES-HTEL antibody ES-x antibody Es22 antibody EST1_HUMAN antibody Esterase 22 antibody Esterase antibody hCE 1 antibody HMSE antibody HMSE1 antibody Liver carboxylesterase 1 antibody Liver carboxylesterase 3 antibody Methylumbelliferyl acetate deacetylase 1 antibody MGC117365 antibody MGC156521 antibody Monocyte carboxylesterase deficiency included antibody Monocyte esterase deficiency included antibody Monocyte/macrophage serine esterase antibody PCE-1 antibody pI 5.5 esterase antibody Proline-beta-naphthylamidase antibody REH antibody Retinyl ester hydrolase antibody Serine esterase 1 antibody Ses-1 antibody SES1 antibody TGH antibody Triacylglycerol hydrolase antibody |
| Molecular Weight(MW) | 62.5kDa(Observed band size: 55kDa) |
| Cellular Localization | Endoplasmic reticulum lumen, Cytoplasm, Lipid droplet. |
WB
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 µg/Lane) Lane 2: Human liver tissue lysate (10 µg/Lane) Lane 3: Human lung tissue lysate (10 µg/Lane) Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Carboxylesterase 1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of U-937 cells labeling Liver Carboxylesterase 1 with Rabbit anti-Liver Carboxylesterase 1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Liver Carboxylesterase 1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of U-937 cells labeling Liver Carboxylesterase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 ICC:1:100 FC:1:500-1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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