| 存储条件 |
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| Product Name | CXCR5 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C-terminal human CXCR5. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:50-1:200 ICC:1:250 FC:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCR5 |
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| Gene Synonyms | BLR1 CD185 MDR15 |
| Gene Full Name | C-X-C motif chemokine receptor 5 |
| Gene Summary | This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| Alternative Names | BLR 1 antibody BLR1 antibody Burkitt lymphoma receptor 1 antibody Burkitt lymphoma receptor 1 GTP binding protein antibody Burkitt lymphoma receptor 1 GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody C X C chemokine receptor type 5 antibody C-X-C chemokine receptor type 5 antibody CD 185 antibody CD185 antibody CD185 antigen antibody BLR 1 antibody BLR1 antibody Burkitt lymphoma receptor 1 antibody Burkitt lymphoma receptor 1 GTP binding protein antibody Burkitt lymphoma receptor 1 GTP binding protein (chemokine (C-X-C motif) receptor 5) antibody C X C chemokine receptor type 5 antibody C-X-C chemokine receptor type 5 antibody CD 185 antibody CD185 antibody CD185 antigen antibody Chemokine (C-X-C motif) receptor 5 antibody Chemokine C X C motif receptor 5 antibody Chemokine CXC motif receptor 5 antibody Chemokine receptor 5 antibody CXC chemokine receptor type 5 antibody CXC R5 antibody CXC-R5 antibody CXCR 5 antibody CXCR-5 antibody Cxcr5 antibody CXCR5_HUMAN antibody MDR 15 antibody MDR-15 antibody MDR15 antibody MGC117347 antibody Monocyte derived receptor 15 antibody Monocyte-derived receptor 15 antibody |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CXCR5 on different lysates with Rabbit anti-CXCR5 antibody at 1/2,000 dilution. Lane 1: Daudi cell lysate, Lane 2: EL4 cell lysate, Lane 3: Neuro-2a cell lysate, Lane 4: C6 cell lysate, Lane 5: Mouse spleen tissue lysate, Lane 6: Rat spleen tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 34 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CXCR5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Neuro-2a cells labeling CXCR5 with Rabbit anti-CXCR5 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCR5 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of Daudi cells labeling CXCR5. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000-1:5000 IHC:1:50-1:200 ICC:1:250 FC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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