| 存储条件 |
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| Product Name | FUBP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human FUBP1 aa 584-633 / 644. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FUBP1 |
|---|---|
| Gene Synonyms | FBP FUBP hDHV |
| Gene Full Name | far upstream element binding protein 1 |
| Gene Summary | The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | DNA helicase V antibody far upstream element (FUSE) binding protein 1 antibody Far upstream element (FUSE) binding protein 4 antibody Far upstream element binding protein 1 antibody far upstream element binding protein antibody Far upstream element-binding protein 1 antibody FBP antibody FUBP antibody Fubp1 antibody FUBP1_HUMAN antibody DNA helicase V antibody far upstream element (FUSE) binding protein 1 antibody Far upstream element (FUSE) binding protein 4 antibody Far upstream element binding protein 1 antibody far upstream element binding protein antibody Far upstream element-binding protein 1 antibody FBP antibody FUBP antibody Fubp1 antibody FUBP1_HUMAN antibody Fubp4 antibody FUSE binding protein 1 antibody FUSE-binding protein 1 antibody HDH V antibody |
| Molecular Weight(MW) | 68kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of FUBP1 on different lysates with Rabbit anti-FUBP1 antibody at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-FUBP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of FUBP1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
FC
Flow cytometric analysis of FUBP1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 FC:1:50-1:100 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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