| 存储条件 |
|---|
| Product Name | CXCL13 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CXCL13 aa 1-109 / 109. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:100-1:500 ICC:1:400 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CXCL13 |
|---|---|
| Gene Synonyms | BLC BCA1 ANGIE BCA-1 BLR1L ANGIE2 SCYB13 |
| Gene Full Name | C-X-C motif chemokine ligand 13 |
| Gene Summary | B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014] |
| Alternative Names | ANGIE antibody ANGIE2 antibody B cell attracting chemokine 1 antibody B cell-attracting chemokine 1 antibody B lymphocyte chemoattractant antibody B-cell chemoattractant antibody B-cell-attracting chemokine 1 antibody B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) antibody BCA-1 antibody BCA1 antibody ANGIE antibody ANGIE2 antibody B cell attracting chemokine 1 antibody B cell-attracting chemokine 1 antibody B lymphocyte chemoattractant antibody B-cell chemoattractant antibody B-cell-attracting chemokine 1 antibody B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) antibody BCA-1 antibody BCA1 antibody BLC antibody BLR1L antibody C-X-C motif chemokine 13 antibody Chemokine (C-X-C motif) ligand 13 antibody Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) antibody Chemokine CXC motif ligand 13 antibody CXC chemokine BLC antibody CXCL13 antibody CXL13_HUMAN antibody SCYB13 antibody Small inducible cytokine B subfamily (Cys-X-Cys motif) member 13 (B-cell chemoattractant) antibody Small inducible cytokine B13 antibody Small inducible cytokine subfamily B member 13 antibody Small-inducible cytokine B13 antibody |
| Molecular Weight(MW) | 13kDa(Observed band size: 12/13 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of CXCL13 on different lysates with Rabbit anti-CXCL13 antibody at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-CXCL13 cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CXCL13 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa transfected with or without CXCL13 cells labeling CXCL13 with Rabbit anti-CXCL13 antibody at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL13 antibody at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:100-1:500 ICC:1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
