| 存储条件 |
|---|
| Product Name | Anti-NDUFA9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NDUFA9 recombinant protein (Position: Q6-I377). Human NDUFA9 shares 78.9% and 76.4% amino acid (aa) sequence identity with mouse and rat NDUFA9, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 500ug/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFA9 |
|---|---|
| Gene Synonyms | CC6 CI39k COQ11 CI-39k MC1DN26 NDUFS2L SDR22E1 |
| Gene Full Name | NADH:ubiquinone oxidoreductase subunit A9 |
| Gene Summary | The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12. [provided by RefSeq, May 2010] |
| Molecular Weight(MW) | 43 kDa(Observed: 36 kDa) |
WB
Western blot analysis of NDUFA9 using anti-NDUFA9 antibody.The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFA9 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody.The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for NDUFA9 at approximately 36 kDa. The expected band size for NDUFA9 is at 43 kDa.
IHC
IHC analysis of NDUFA9 using anti-NDUFA9 antibody . NDUFA9 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NDUFA9 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of NDUFA9 using anti-NDUFA9 antibody and anti-Beta Tubulin antibody. NDUFA9 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-NDUFA9 Antibody at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody(green)and Dylight550-conjugated Anti-mouse IgG Secondary Antibody(red)were used as secondary antibody.
FC
Flow Cytometry analysis of U87 cells using anti-NDUFA9 antibody . Overlay histogram showing U87 cells stained with A07420-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA9 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat antirabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FC:1:50-1:200 |
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| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
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