| 存储条件 |
|---|
| Product Name | Sp7 / Osterix Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Sp7 aa 1-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| WB:1:2000 IHC:1:200-1:1000 IF:1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Sp7 |
|---|---|
| Gene Synonyms | Osx |
| Gene Full Name | Sp7 transcription factor |
| Gene Summary | Predicted to enable DEAD/H-box RNA helicase binding activity; DNA-binding transcription activator activity, RNA polymerase II-specific; and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in hematopoietic stem cell differentiation; positive regulation of stem cell differentiation; and positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within osteoblast differentiation. Predicted to be located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in osteogenesis imperfecta type 12. Orthologous to human SP7 (Sp7 transcription factor). [provided by Alliance of Genome Resources, Apr 2022] |
| Alternative Names | MGC126598 antibody Osterix antibody Osx antibody Sp 7 antibody SP7 antibody Sp7 transcription factor antibody SP7_HUMAN antibody Transcription factor Sp7 antibody Zinc finger protein osterix antibody |
| Molecular Weight(MW) | 45kDa |
| Cellular Localization | Nucleus. |
| Entrez Gene | 300260 |
|---|
WB
Western blot analysis of Sp7 / Osterix on Saos-2 cell lysates (30 µg/Lane) with Rabbit anti-Sp7 / Osterix antibody at 1/2,000 dilution. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue with Rabbit anti-Sp7 / Osterix antibody at 1/1,000 dilution. he section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF
Immunofluorescence analysis of paraffin-embedded E14.5 mouse embryo tissue labeling Sp7 / Osterix with Rabbit anti-Sp7 / Osterix antibodyat 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:2000 IHC:1:200-1:1000 IF:1:500 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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