| 存储条件 |
|---|
| Product Name | GFP-Tag Monoclonal Antibody(Mix) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Protein of GFP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| WB:1:5000 IP:1:200 IF:1:50-200 | |
| Species Reactivity | A wide range of other species |
| Purification | Affinity purified |
| Gene Full Name | Green Fluorescent Protein |
|---|---|
| Gene Summary | Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues. |
WB
Western blot analysis of GFP transfected Hela, diluted at 1) 1:5000 2) 1:10000
IP
ADAR1 links R-loop homeostasis to ATR activation in replication stress response. NUCLEIC ACIDS RESEARCH Lei Shi WB,CoIP Human HeLa cell
ICC/IF
Cooperative effects of SIRT1 and SIRT2 on APP acetylation. AGING CELL Liu Cao IF Human 1:1000 HEK293 cell| Application Notes | WB:1:5000 IP:1:200 IF:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
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