| 存储条件 |
|---|
| Product Name | DUSP3 Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human DUSP3. AA range:136-185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500 - 1:2000 IHC:1:100-300 ELISA:1:20000 IF:1:50-200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DUSP3 |
|---|---|
| Gene Synonyms | VHR |
| Gene Full Name | dual specificity phosphatase 3 |
| Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 21kDa |
| Cellular Localization | Nucleus |
WB
Western Blot analysis of HepG2 cells using DUSP3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
WB
Western blot analysis of lysate from HepG2 cells, using DUSP3 Antibody.
IHC
Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100| Application Notes | WB:1:500 - 1:2000 IHC:1:100-300 ELISA:1:20000 IF:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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